Generator
Part:BBa_K1510009:Experience
Designed by: Chien-Yung Huang Group: iGEM14_NYMU-Taipei (2014-10-06)
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Applications of BBa_K1510009
Using yebF at the N-terminal of desired sequence as recombinant protein would help exporting it extracellularly. Especially useful when common experimental strains, such as K-12 are used, since they don't naturally secrete protein outside.
User Reviews
UNIQ0b4341f5179782ac-partinfo-00000000-QINU To test if our yebF works, we fuse RFP at the C-terminal of the protein, and test if the signal protein works by examing the fluorescence intensity in the culture supernatant. This is our result under Multimode microplate reader: UNIQ0b4341f5179782ac-partinfo-00000001-QINU